MEK2 Antibody PA5-118046 from Thermo Fisher Scientific

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Western Blot using MEK2 Polyclonal Antibody (Product # PA5-118046) at 1:2,000 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: HeLa Whole Cell Lysate, Lane D: A549 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa. (We are unsure of the identity of these extra bands).
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Immunofluorescence staining of MEK2 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with MEK2 Polyclonal Antibody (Product # PA5-118046, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Supplier Page from
Thermo Fisher Scientific for
MEK2 Antibody

Thermo Fisher Scientific's MEK2 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunocytochemistry, Immunoprecipitation, and Western Blot. The MEK2 Antibody was generated using MAP2K2 as the antigen and it reacts with Homo Sapiens, and Human.

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ICC/IF

MEK2 Antibody from Thermo Fisher Scientific

Thermo Fisher Scientific

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MEK2 Antibody from Thermo Fisher Scientific

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